Kap bra ana kap chye ai dumsu n-gup n-gu ana zinli hpe ninghkap ai ELISA sawk sagawn ai jak

Ga shadawn:VD105E
IBRV Ab Elisa
Laksan: serum

Sanglang dan ai lam

Laksan masat masa

BVDV Ab Elisa

Yaw shada ai lang na: Kap bra anaDusat n-gup ana ana zinliAntibody ELISA hpe sawk sagawn ai jak hpe lang nna .kap chye ai n-gup n-gu ana hpe dawdan uvirus hpe ninghkap ai n-gun hpe dumsu sai hta in vitro hta mu lu ai.

Sample: sai chyup

Jaw ai arung arai ni:

Infectious Bovine Rhinotracheitis Virus Antibody ELISA Detection Kit

Chyam dinglik ai ladat hte lachyum shaleng ai lam

STEP 1 Chyam dinglik ai ladat

1.1 Hka shin na matu hkyen lajang ai lam

Wbungli galaw ai myit maju jung ai lam washing ntsin:N lang shi yang, kashin ai ntsin hpe nta kata na nbung katsi ai (20-25℃) de bai gram lajang nna, maren mara shinggrup nna, maren mara byin hkra galaw ra ai. Dai hpang, san seng ai hka hte hka ja ai hka hpe 20 lang garan nna (ga shadawn, san seng ai hka 95ml hta 5ml hka ja ai hka hpe bang nna), atsawm sha kayau nna, hka ja ai hka hpe 2-8℃hta 7 ya tup tawn da mai ai.

1.2 Sample hpe hkyen lajang ai lam

Dusat sai ting hpe la nna, 4000rpm hta minit 10 daram centrifuge galaw nhtawm, npu na sai hpe shaw la ai. Serum hpe mung hemolysis n galaw ai sha shingra tara hku nna n-gun jat wa shangun lu ai.

Chyam yu na serum hpe serum hka ja ai plate hta 50 lang hka ja na (ga shadawn: chyam yu na serum 3ul hpe 147ul sample diluent hta bang na).

Myit yu na lam: Negative hte positive hpe hkang ai lam ni hpe n-gun n jaw ra ai

1.3 Chyam dinglik ai ladat

1Enzyme label Microplate hpe la nna (sample jahpan hte maren, garan nna kalang lang lang mai ai), enzyme label Microplate de 100ul diluted serum hpe bang nna, negative control hte positive control a matu hka htung lahkawng langai hpra hpe tawn da nna, respective hka htung de 100ul bang dat u. Sample hpe hka htung kata kaw loi loi hte shinggrup nna, sealing plate film hpe lang nna, plate hpe ka-up nna, 37℃hta minit 30 daram shinggrup da u.

2Sealing plate film hpe hpaw nna, plate hka htung kata na ntsin hpe shinggrup kau nhtawm, hka htung langai hpra hta 300μl working concentration kashin ntsin bang nna, hka htung kata na ntsin hpe kabai kau u. Lahta na lahkam ni hpe 5 lang bai galaw nna, hpang jahtum san seng ai absorbent paper ntsa hta pat dry galaw u.

3Diluted enzyme conjugate:Enzyme n-gun (80×) hpe 80 lang n-gun dat (ga shadawn: 10ul enzyme n-gun dat + 790ul sample diluent, ra ai n-gun hpe chyam dinglik ai sample jahpan hte maren sawn la nna, atsawm sha kayau nna, garan tawn da na.

4Hka htung langai mi hta Enzyme Conjugate 100μl bang nna, plate hpe ka-up nna, 37℃hta minit 30 daram shingbyi tawn da na.

5Enzyme plate hpe kashin ai shaloi, lawu na hte maren sha galaw ra ai 2.

6Hka htung langai mi hta 100μl substrate solution bang nna, plate hpe ka-up nna nhtoi kaw na 37℃tsan ai shara kaw minit 10 daram shingbyi tawn da na.

STEP 2 Mahtai lachyum shaleng ai lam

Hka htung langai hpra a OD450 manu hpe hka htung 96 rawng ai microplate hti ai jak hta hti yu ai.

2.1 Validation Negative control a OD450nm manu gaw < 0.25 rai nna, kaja ai control a OD450nm manu gaw 0.6 hta grau kaba ai (sh) maren sha rai yang, chyam dinglik ai lam a mahtai gaw kaja ai. N rai yang, chyam dinglik ai lam hpe bai galaw ra ai.

2.2 Jahpan ladat .

Sample langai hpra a matu, gram lajang da ai sample hte control manu ni hpe lang nna lawu na hte maren S/P hpe sawn la u:

product-554-49

2.3 Mahtai hpe lachyum shaleng ai lam

S/P < 0.2 rai yang, n kaja ai ngu dawdan ai.

S/P > 0.3 rai yang, kaja ai ngu dawdan ai.

0.2 S/P hta n law ai (sh) maren sha rai yang 0.3 hta n law ai (sh) maren sha rai yang, myit ung ang hpa re ngu dawdan nna, bat mi na ai hpang bai sample la nna chyam yu na matu hpaji jaw ai.

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Moi na: Bovine Virus Ana Zinli hpe ninghkap ai Elisa Kit

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