Bovine Virus Ana Zinli hpe ninghkap ai Elisa Kit

laksan masat masa galaw ai lam

 

Chyam dinglik ai lam ni & lachyum shaleng ai lam

 

STEP 1 Chyam dinglik ai lam

1.1 Hka shin hkyen lajang ai lam

Wshing nrai, myit maju jung ai lam washing ntsin:N lang shi yang, wash hparan ladat hpe nta kata na nbung katsi ai lam (20-25℃) de bai gram lajang nna, langai sha re ai hku galaw na matu maren mara shinggrup ra ai. Dai hpang, hka hpe san seng hkra galaw da ai hka hte htam 20 daram hka ja la u (ga shadawn, 5ml concentrated waling hka hpe 95ml san seng ai hka hta bang dat u), hka hpe atsawm sha kayau nna, diluted waying liquid hpe 2-8℃hta 7 ya tup tawn da mai ai.

1.2 Sample hpe hkyen lajang ai

① Dusat sai hkum hkrang ni hpe 4000rpm hta minit 10 daram centrifuge galaw nna, supernatant hpe shinggyin la lu ai. Serum hpe mung shingra tara hku nna hemolysis n galaw ai sha coaglate galaw mai ai.

② Serum sample 1:50 (147ul sample diluent de 3ul sample hpe bang nna, atsawm sha kayau dat u.

Myit yu na lam: Negative hte positive control ni hpe mung dilute galaw ra ai.

1.3 Enzyme hku nna hkyen lajang ai lam

1:250 (2ul enzyme conjugate(x100) hpe 498ul sample diluent de bang nna, atsawm sha kayau nna, enzyme conjugate hpe dip dat u.

1.4 Chyam dinglik ai lam

†Bracroplate hpe enzyme ngu ai label hpe ka bang u ( sample ni a jahpan hte maren, garan ginhka nna lang mai ai), 100ul hpe enzyme label hku nna Microplate ngu ai laika hta bang nna, hka htung lahkawng hpe negative hte positive sample ni a matu masat da nna, hka htung hpe n kaja ai hte kaja ai hku hkang zing ai ni a matu langai hpra hpe 100ul bang u. Hka htung kata na sample hpe loi loi hte shake galaw nna, sealing plate film hpe lang nna plate hpe ka-up nna, 37℃hta minit 30 daram n-gun dat dat u.

②Sinped plate film hpe garan nna, hka htung kata na hka hpe shake off galaw nna, hka htung langai hte langai hta bungli galaw ai n-gun hpe kashin ai ntsin hpe 300μl bang nna, hka htung kata na ntsin hpe kabai kau u. Lahta na lahkam ni hpe 5 lang bai galaw nna, hpang jahtum e san seng ai absorbent paper ntsa kaw pat ai.

③Add 100μl Enzyme Conjugate hpe hka htung langai hte langai hpe shinggrup nna, plate hpe shinggrup nna, 37℃hta minit 45 daram n-gun dat bang u.

④ Sealing plate film hpe hpaw nna, hka htung kata na hka hpe shake off galaw nna, hka htung langai hte langai hta bungli galaw ai myit masin hpe kashin ai ntsin hpe 300μl bang nna, hka htung kata na ntsin hpe kabai kau u. Lahta na lahkam ni hpe 5 lang bai galaw nna, hpang jahtum e san seng ai absorbent paper ntsa kaw pat ai.

100μl substrate ntsin hpe hka htung langai hte langai hta bang nna, plate hpe ka-up nna, nhtoi kaw na 37℃tsan ai shara kaw minit 10 daram bang da u.

⑥Add 50μl hpe hka htung langai hte langai hpe jahkring kau nna, minit 5 laman mahtai hpe dawdan u.

 

STEP 2 mahtai hpe san ai

 

Hka htung langai hte langai a OD450 manu hpe 96-well microplate hti ai wa a ntsa hti ai.

2.1 Validation

Negative control a OD450nm manu gaw .<0.1 and the average OD450nm value of positive control /the average OD450nm value of negative control was ≥3, the experimental results were valid. Otherwise, the test should be repeated.

2.2 Ladat Ladat

Sample langai hpra a matu, S/P hpe gram lajang da ai sample hte control manu ni hpe lang nna lawu na hte maren sawn la u:

2.3 Mahtai Htai ai lam

S/P<0.25, it is judged as negative.

S/P 0.35 hta grau kaba ai (sh) maren sha rai yang, kaja ai ngu masat da ai.

0.25 S/P hte maren n law ai (sh) maren sha re.<0.35, it is judged as suspicious, and suggest to repeat sampling and test one week later.

 

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